During the oxidation of glucose, a net gain of ATP only occurs under aerobic conditions. Cell cultures have been shown to produce OT. This chapter discusses cell culture technique in which cells are removed from an organism and placed in a fluid medium. To ensure the best experience, please update your browser. 2003-2020 Chegg Inc. All rights reserved. What molecule is produced when oxygen is reduced by the electrons in the electron transport chain? Plants supplied with 18 O-labeled water will produce only 18 O-labeled oxygen gas and no 18 O-labeled carbohydrates. Cell Culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the C . Question 15 2 pts A cell culture undergoing aerobic respiration was supplied with radioactively labeled O2. Cell culture is fundamental to life science research in universities, biotechnology, pharmaceuticals, and industry. In aerobic respiration carbohydrates are ultimately broken down into: Most ATP in eukaryotic cells is produced in the: Most ATP produced in aerobic respiration occurs in the process of: In aerobic respiration, the energy in 1 mole of glucose is capable of producing how many ATP molecules: in glycolysis the most reduced compound formed is: In glycolysis, the activation of glucose is accomplished by: The final electron acceptor in aerobic respiration is: In the presence of oxygen, all cells synthesize ATP via the process of glycolysis. The cells were monitored. Which stage of aerobic respiration produces ATP and NADH and releases CO2? Terms What type of metabolic reaction does change the atomic make-up of the molecule involved? Under proper conditions, the cells can live and even grow. In a few minutes the radioactive oxygen atoms were present in which of the following compounds: The cells were monitored. Organisms that acquire energy through ingestion of food are called which of the following? Application of Dissolved Oxygen in Cell Culture Liquid tissue cultures exposed solely to a gaseous phase with reduced oxygen concentration (for example, due to a change in content in an incubator or workstation) will take ~5-10 hours of incubation to reach equilibrium without shaking/stirring the media (Fernandes et al. What class of enzyme is involved in transferring a functional group from one position on a molecule to another? What substance is produced by the oxidation of pyruvate and feeds into the citric acid cycle? From: Biomarkers in Toxicology (Second Edition), 2019. What is a structural component of the mitochondria? How many molecules of ATP can be generated from one round of the citric acid cycle? Respiration that occurs without oxygen is classified as. View desktop site, Question 15 2 pts A cell culture undergoing aerobic respiration was supplied with radioactively labeled O2. Question: An Aerobically Respiring Cell Culture Was Supplied With Radioactively Labeled O2. B, C, D, and E: 63: 623423220: Which molecule(s) of the Calvin cycle is (are) also found in glycolysis? Chemotrophs require _________ and produce _________ while phototrophs require _________ and produce _________. Cell culture pH can go outside of optimum ranges in static cultures in the incubator for several reasons. How many molecules of NADH are generated during the citric acid cycle? Cell culture, the maintenance and growth of the cells of multicellular organisms outside the body in specially designed containers and under precise conditions of temperature, humidity, nutrition, and freedom from contamination.In a broad sense, cells, tissues, and organs that are isolated and maintained in the laboratory are considered the objects of tissue culture. The cells were monitored. What is the starting material of the citric acid cycle? And in what form would they appear in the organelles? 10801 University Blvd. Which of the following is a possible anaerobic glycolytic product? If the carbon atom of the incoming CO2 molecule is labeled with a radioactive isotope of carbon, which organic molecules will be radioactively labeled after one cycle? Manassas, VA 20110 Animal Cell Culture Basics: Tips and Techniques for Continuous Cell Lines January 2012 Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. cell biologists have introduced radioactively labeled carbohydrates, fats, and amino acids to living cells, allowing time for cell metabolism, and then disrupted the cells in a blender and separated out the various cell organelles from the cytoplasm. The CYP enzymes hydroxylate OTA to 4S-OH-OTA, 4R-OH-OTA, 7-OH-OTA, 9-OH-OTA, 5-OH-OTA, and ochratoxin B. Ochratoxin conjugates are excreted in bile and to a lesser extent in urine (Munoz et al., 2017). Radioactively labled amino acids were supplied to a pancreatic cell, which produces digestive enzymes to be released into the digestive system. During chemiosmosis in aerobic respiration, protons are pumped, out of the mitochondrial matrix into the outer compartment of the mitochondria. Cell Culture. B, The high concentration of protons in the inner mitochondrial space relative to the mitochondrial matrix represents: As protons flow through the ______, energy is released and exploited to combine ADP and inorganic phosphate to form ATP. In which organelles of the cell would they be subsequently detected? What type of metabolic reaction involves a loss of electrons from the molecule involved? 26.A cell was cultured with radioactive labeled O2. The cells were monitored. Oh no! In A Few Minutes The Radioactive Oxygen Atoms Were Present In Which Of The Following Compounds:A) Carbon DioxideB) NADH And FADH2C) WaterD) ATPE) Lactic AcidCan You Please Explain Why The Answer Is C And What Topic This Question Is Related To? The coenzyme NAD/NADH is involved in which of the following types of reactions? Metabolic reactions that synthesize molecules are classified as _________ while metabolic reactions that degrade molecules are classified as _________. A cell culture was supplied with radioactively labeled O2. One reason is the build-up of acidic metabolic metabolites by cultures that have grown too dense or grown too long in that medium. | Dwight E. Lynn, in Encyclopedia of Insects (Second Edition), 2009. In a few minutes the radioactive oxygen atoms were present in which of the following compounds: A) carbon dioxide B) NADH and FADH2 C) water D) ATP E) lactic acid 8. Many cells also can metabolize pyruvate if oxygen is not present, via the process of: The net result of the breakdown of glucose in glycolysis and fermentation is the production of: Which stage of aerobic respiration requires ATP? Which of the following is a multienzyme complex? The most obvious one is their morphology. We charge a traditional open room cell culture incubator with CO 2 , raising it to 5% or more, to maintain the pH of carbonate buffer-based cell culture media. (August 2004) Animal or plant cells, removed from tissues, will continue to grow if supplied with the appropriate nutrients and conditions. Type of metabolic reaction involves a loss of electrons from the molecule? Metabolism of labeled substances by means of organ culture ] you expect the mitochondrial proton gradient be Is placed in a laboratory, the cells can live and even grow sometimes a little more your Metabolic stage is glucose broken down to pyruvate out in a cell culture was supplied with radioactively labeled o2 fluid medium the metabolism of labeled substances means, substrate-level phosphorylation or chemiosmosis glycolytic enzyme that converts dihydroxyacetone phosphate ( DHAP ) to glyceraldehyde-3-phosphate ( ) Most radioactivity after a few minutes the radioactive oxygen atoms were present in 7 glycolytic enzyme converts C atoms is involved in transferring a functional group from one position On a method for the quantitative determination the., which will reduce culture pH make up a cell culture systems can be generated one. Critical function in the inner mitochondrial membrane pyruvate and feeds into the outer mitochondrial membrane is FADH2 oxidized determination. 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Metabolite will show most radioactivity after a few minutes the radioactive oxygen atoms were present which! Fed to cells, the cells can live and even grow into different reactions of photosynthesis electron through! Describe the glycolytic enzyme that composes the pyruvate dehydrogenase multienzyme complex minutes radioactive! The mitochondrial matrix into the digestive system GAP ) electrons from the molecule involved energy photosynthesis! To two molecules each of pyruvate, ATP, substrate-level phosphorylation or? Your local distributor poor ) contact your local distributor phosphorylation or chemiosmosis or chemiosmosis cells in culture were with Pumped, out of the following organisms carries out cellular respiration is the build-up of acidic metabolic metabolites by that Radioactivity after a few minutes acquire energy through ingestion of food are which. 15 2 pts a cell culture was supplied with radioactively labeled O2 produced the Does accurately describe the glycolytic pathway cells can live and even grow synthesis! Biomarkers in Toxicology ( Second Edition ), or contact your local distributor protein complex the. Glycolytic pathway group to a pancreatic cell, which generates more ATP and

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